MD A7: Identification of autoantibodies contributing to the break of immunotolerance in immunization induced MMP mouse model
Mucosal pemphigoid (MMP) is one of the bladder-forming autoimmune diseases with predominant involvement of the mucous membranes. Autoantibodies target components of the basal membrane. In particular, it shows an activity directed against the protein laminin-332 which is associated with an increased risk of developing malignant tumours.Patients with MMP have been shown to have an HLA polymorphism whose influence on the development of the disease is still unclear. Tu study this mechanism, samples from the established MMP mouse model designed for anti-laminin 332 MMP are used. This shows great similarity to characteristics of human disease expression.Samples of this mouse model are used to determine the genetic impact on disease development, as well as the influence of antibody subclasses. To a certain extent, conclusions can be drawn about the underlying pathophysiology, in particular the development of antibodies against different epitopes or proteins.To achieve these objectives, samples in the form of serums and biopsies are collected from mice with three different genetic backgrounds. These were immunized with two different fragments of the murine laminin-332 alpha chain.Epitopes and proteins that can act as auto antigens in MMP are analyzed with the help of western blot and immunoprecipitation.In order to investigate the question of epitope spreading, nearby structural proteins (mCOL7, mCOL17) are included in the study. The autoantibodies are analyzed by ELISA regarding their subclasses. This examination is continued in the form of indirect and direct immunofluorescence.
- Projects
- 1st Generation
- A: Defining Autoimmune Pre-Disease
- B: Targeting of Autoimmune Pre-Disease
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- MDs
- MD A1 - Investigation of the influence of specific CDK inhibitors on neutrophil activation
- MD A2 - Conception of an anatomical expression of landscape of target antigens in autoimmune blistering dermatoses as markers for lesion formation
- MD A3 - Structural characterization of skin-directed autoantibodies and their interaction with the antigen to gain insights into autoimmune pre-disease
- MD A4 - Do interactions between AT1R autoantibodies derived from patients with systemic sclerosis and endothelial cells lead to endothelial dysfunction?
- MD A5 - Optimization and exploitation of a 3D model of human skin for translational use
- MD A6- To study vasculopathy in systemic sclerosis
- MD A7- Identification of autoantibodies contributing to the break of immunotolerance in immunization induced MMP mouse model
- MD A8- The role of AT1R antibodies and extracellular vesicles in mediating endothelial dysfunction in systemic sclerosis with pulmonary arterial hypertension
- MD A9- Impact of glycosylation on IgG4-induced signaling in neutrophils
- MD A10- Testing a new single chain variable fragment for pemphigus foliaceus in the human skin organ culture model
- MD A11- Impact of glycosylation on IgG3-induced signaling in neutrophiles
- MD A12- Screening for inhibitors to prevent keratinocytes dissoziation
- MD A13- Investigation of the local and systemic complement activation in bullous pemphigoid
- MD A14 - Impact of different subclasses on immune complex-induced signaling in neutrophils.
- MD A15 - Novel target antigens as inducers of autoimmunity of autoimmune bullous dermatoses
- MD A16 - Identification of the major epitope of the BP180 ectodomain recognized by serum IgA autoantibodies of patients with pemphigoid diseases –IgA autoantibodies as prognostic marker?
- MD A17 - Autoantibody-mediated effects on endothelial and immune cell signaling in systemic sclerosis
- MD A18 - Molecular and cellular characterization of pre-autoimmune effects induced by aging in mice
- MD B1 - Testing the effect of kinase inhibitors in the human skin organ culture model
- MD B2 - Cigarette smoking-induced autoantibodies
- MD B3 - Contribution of taurine, hypoxanthine, vitamin B5 and B6 in the pathomechanism of pemphigus vulgaris
- MD B4 - The influence of prednisolone treatment on split formation in the human skin organ culture model for pemphigus vulgaris
- MD B5 - Molecular characterization of the pre-autoimmune effects of Western diet in healthy mice
- MD B6 - Testing the effect of kinase inhibitors in the human skin organ culture model for pemphigus foliaceus
- MD B7 - Testing the Effect of Kinase Inhibitors in the Human Skin Organ Culture (HSOC) Model for Pemphigus Foliaceus
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